Mean and standard deviations were determined from triplicate analyses independently grown cultures. These were: 3. A cell volume of 1 fl was used to calculate [P] total and [D] total from the number of protein assemblies per cell i. DNA occupancy by each sensor was normalised from zero to one using the minimum and maximum DNA occupancy values. The equilibrium concentration of free metal i. This was solved, via a quadratic equation, using initial concentrations of metal and buffer of 0.
This gives the fractional occupancy of DNA at a buffered metal concentration of 5. Complete column 'N' as described in step 8. The data supporting the findings of this study are available within the article and its Supplementary Information files, or from the corresponding author upon request. Guerra, A. Metal site occupancy and allosteric switching in bacterial metal sensor proteins. Gilston, B. Structural and mechanistic basis of zinc regulation across the E.
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Crystal structure of the Iron-dependent Regulator IdeR from Mycobacterium tuberculosis shows both metal binding sites fully occupied. Characterization of the metal receptor sites in Escherichia coli Zur, an ultrasensitive Zinc II metalloregulatory protein. Biochemistry 40 , — Ammendola, S. Petrarca, P.
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